Why were there less colonies on the transform Nutrient Agar amp plate than on the transform Nutrient Agar plate when cells were plated from the same microtube of cells? A. The uptake rate of the plasmid varies. Not all the bacteria were transformed with enough copies of the ampi n resistance plasmid to neutralize the ampicillin. B. All of the answer choices are correct C. The colonies closer to the antibiotic discs were killed, while the farther away colonies survived.