You have a tube with 5 ml of infected blood that has a concentration of bacteria at 10^8 cells per ml. You wish to make a serial dilution of the blood so when you take 100 ul of the last dilution and spread it on a Petri dish you will have between 30-300 colonies growing on the plate. You have plenty of empty 1.5 ml microfuge tubes, a bottle of sterile water, a p1000 pipettor (l00-l000ul) and tips, for the dilutions, but you only have ONE Petri dish. Clearly diagram how you would make these dilutions, add them to the plate, and prepare it for incubation. Also show exactly how many colonies you would expect to have on the plate when using your procedure. Don’t forget to clearly label the amounts measured and transferred. One of your goals is to make these dilutions with the fewest possible numbers of tubes and materials. Points will be deducted for unnecessary steps.
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Question: You have a tube with 5 ml of infected blood that has a concentration of bacteria at 10^8 cells pe…
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