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Question: You have isolated a new species of bacterium, Mustget caffeinum, from a compost heap that was hea…

by | Dec 5, 2023 | Posted Questions

Question: You have isolated a new species of bacterium, Mustget caffeinum, from a compost heap that was hea...

You have isolated a new species of bacterium, Mustget caffeinum, from a compost heap that was heavily enriched with coffee grinds. you discover that it can grow using caffeine as its only carbon source but seems to grow better on other carbon sources. You decide to compare its growth rate in defined minimal media(MM) supplemented with either sucrose or caffeine as the sole carbon source. You inoculate M. caffeinum into both MM-caffeine and MM-sucrose at 8 AM. Because this species is filamentous, its growth is most accurately measured by dry weight. At regular intervals throughout the day, you vigorously mix the remove 10 mL, filter the cells onto a pre-weighed culture, and weigh the disk, dry the disk in a 40 degree C oven, and weight the disk. You graph the calculated net weight of the cells over time and determine that both cultures reached exponential phase by 9 AM. You use the following measurements to calculate the generation times. Which carbon source does M. caffeinum prefer? Show your work.

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