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Question: You have plasmid DNA at a concentration of 193 ng/ml. From this sample 3 mu l are added to 100 mu…

by | Dec 5, 2023 | Posted Questions

Question: You have plasmid DNA at a concentration of 193 ng/ml. From this sample 3 mu l are added to 100 mu...

You have plasmid DNA at a concentration of 193 ng/ml. From this sample 3 mu l are added to 100 mu l of competent cells. After the appropriate steps 400 mu l of nutrient broth are added and the cells incubated for one hour. A 100 mu l sample is spread on a plate containing selecting antibiotic. Following overnight incubation 243 colonies are present on the plate. a) How much picogram (pg) of DNA was added to the competent cells? b) How much picogram (pg) of DNA was in the sample spread on the plate? c) What is the transformation efficiency? Remember: Take note of the units. Also, make sure to note the formula of calculating transformation efficiency from lab hand out. SHOW YOUR CALCULATIONS.

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