You move on to a new project. This time, you’re studying the signal sequences on various RNA polymerase II so bun its. You add the gene sequence for GFP to the N-terminus of the main RNA polymerase II subunit so that it is visible under the microscope. It is important to know that the nuclear localization signal (NLS) of this protein is located in the middle of the amino acid sequence (not at the extreme N-terminus and not at the extreme C-terminus). a. Suppose you mutate the NLS of RNA polymerase so that it no longer interacts with importin. i. Where will you see RNA polymerase (green due to GFP) in normal (unmutated) cells’ Explain. ii Where will you see the RNA polymerase in cells containing the mutant form? Explain. iii. What will happen to transcription in the cells containing this mutant? Explains your answer. b. Suppose you mutate the NLS like you did in part a and also add an ER-SS to the N-terminus, before GFP. i. Where do you expect to find RNA polymerase In these cells? Explain. ii. What will happen to transcription in cells containing this replacement mutant’ Explain. c. Suppose you take the normal protein (NLS is not mutated) and add an ER-SS to the N-terminus, before GFP. i. Where will you see RNA polymerase when you look at cells under the microscope? Explain. ii. What will happen to transcription in cells containing this mutant? Explain.