You set up your PCR reaction to run:

PARENTAL 1 (P1) 5′-ATCGATAAACGTGTGACCCTGGC-3′

PARENTAL COPY 1 (PC1) 3′-TAGCTATTGCACACTGGGACCG-5′

COPY PC1 (CPC1) 5′-ATCGATAACGTGTGACCCTGGC-3′

PARENTAL 2 (P2) 3′-TAGCTATTGCACACTGGGACCG-5′

PARENTAL COPY 2 (PC2) 5′-ATCGATAACGTGTGACCCTGGC-3′

COPY PC2 (CPC2) 3′-TAGCTATTGCACACTGGGACCG-5′

2.Based on what you know about DNA synthesis, fill in how many
strands of each of the following you would have after the first
cycle below. For this exercise, assume that any complementary
strands of copy PC (CPC) are considered as CPCs as well.

3. How many strands of each of the following you would have
after the second cycle? How many target sequences would you
have?

4.How many strands of each of the following you would have after
the third cycle? How many target sequences would you have?

5.How many strands of each of the following you would have after
the fourth cycle? How many target sequences would you have?

6. Your PCR has gone through ten cycles, based on what you know
about DNA synthesis, fill in how many strands of each of the
following you would have after the tenth cycle below.

7.Graph how many total sequences you would have after four
rounds of extension. Remember to include labels.