Your friend is trying to amplify a DNA sequence using PCR, but
he didn’t feel too confident about designing the primers, so he
ordered two different sets of primers (4 primers total).
Unfortunately, neither set produced a product. Below are the
primers that he used and the DNA to be amplified:

DNA template:

           
5’ ATTCGGACTTG—-(750 bases to amplify)—-GTCCAGCTAGAGG 3’

           
3’ TAAGCCTGAAC————————————–CAGGTCGATCTCC
5’

Primer pair #1      (a) 5’ GGACTTG
3’           
(b) 5’ GTCCAGC 3’

Primer pair #2      (a) 5’ TTCAGGC
3’           
(b) 5’ AGCTGGA 3’

a) With the DNA denatured as below, show where primer pair #1
should anneal. Would this end up in a PCR product, why or why
not?

5’ ATTCGGACTTG—-(750 bases to amplify)—-GTCCAGCTAGAGG 3’

3’
TAAGCCTGAAC————————————–CAGGTCGATCTCC
5’

b) With the DNA denatured as below, show where primer pair #2
should anneal. Would this end up in a PCR product, why or why
not?

5’ ATTCGGACTTG—-(750 bases to amplify)—-GTCCAGCTAGAGG 3’

3’
TAAGCCTGAAC————————————–CAGGTCGATCTCC
5’