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Question: Your friend is trying to understand how the steroid hormone cortisol affects kidney, liver and bo…

by | Dec 5, 2023 | Posted Questions

Question: Your friend is trying to understand how the steroid hormone cortisol affects kidney, liver and bo...

Your friend is trying to understand how the steroid hormone cortisol affects kidney, liver and bone cells by examining a sec of cell lines derived from kidney cells liver cells, and bone cells. She gets the following result when she examines her cell lines for expression of the cortisol receptor on a Western blot depicted in the figure (A), to the right. Unfortunately, after she performs the Western blot experiment, she mixes up her cell line and is not sure which line comes from which cell type. She labels the cell lines, A, B, and C and then examines the activity of a cortisol-responsive reporter gene’s expression in the presence and absence of cortisol and collects the data depicted in Figure B. a) Where in the cell would you expect the cortisol receptor detected in liver and kidney cells to be located? Explain. b) Which cell line tested in Figure B do you think is the bone cell line, A, B, or C? Explain. Provide a possible explanation for why cell line B and cell line C could show differences in their cortisol response.

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