PRELAB EXERCISE 15 Read pages 315-324 of the lab manual and answer the following questions DNA is a polymer of ____________________ linked by ___________________ bonds.
What do restriction endonucleases do?
Which organisms have restriction enzymes and what purpose do they serve?
Give an example of a palindrome sequence that consists of 4 bases.
What is the difference between sticky ends and blunt ends?
If a circular molecule of DNA has 2 restriction sites how many fragments will be seen on a gel?
If a linear molecule of DNA has 2 restriction sites how many fragments will be seen on a gel? What is agar?
If electric current is applied to DNA, is it going to travel to the negative or the positive electrode and why?
If a molecule of DNA is linear, nicked circular, or supercoiled, place the 3 forms in the order they will move in a gel, from fastest to slowest:
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